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“Wound Repair” new Academic Presentations

We made an academic presentation about the effect of KA21 relating to “Wound repair” at 1st Japanese Society of Veterinary Anesthesia and Surgery Online Lecture 2020. This presentation was joint presentation with Tokyo University of Pharmacy and Life Sciences.

Effects of  Agaricus brasiliensis KA21 Relating to Wound Repair

Background

Agaricus brasiliensis (=Agaricus) is a medicinal mushroom native to Brazil referred to by the scientific name of Agaricus brasiliensis, Agaricus blazei, Agaricus subrufescens, etc. The safety and functional components of Agaricus differ depending on strain, cultivation conditions, and production origin.

To date, we have researched about Agaricus cultivated outdoor in Brazil, KA21 strain (=KAOD) and confirmed KAOD’s effects to anti-fungal infection, improve diabetes and protect liver. Outdoor cultivated Agaricus has stronger liver protective effect than green house cultivated Agaricus.

During this latest research, while analyzing the physiological effects of KAOD, we confirmed the veterinary field effects of KAOD; improving fur and skin condition, hair growth effects related skin. Then, we will report the ability to wound repair.

Test Design

We acclimatized test model mice by feeding them AIN93G free of βG for a period of one week. After this, we switched to feeding the mice AIN93G (as the control group), KAID (A. brasiliensis KA21 strain cultivated indoor) and KAOD  (A. brasiliensis KA21 strain cultivated outdoor). One week after switching to the three types of food, we performed the below processes.

Wound repair model

With the mouse under anesthetic, we shaved a part of the fur on its back, then removed some of the skin using a biopsy punch for dermatology. We measured the size of the wound 3/6/9/12 days later, as well as the gene expression levels for TGF-β, Collagen Type 1, and Collagen Type 3.

Results

Wound size

Repair of the wound was confirmed in the order of KAOD>KAID> control group(*Significant difference existed between the KAOD and control groups)

TGF-β, Collagen Type 1,Collagen Type 3 were produced in the order of KAOD>KAID> control group

Considerations

It was suggested that KAOD may contribute to faster wound healing through promoting the production of TGF-β, Collagen Type 1, and Collagen Type 3. It has been reported that the β-glucan contained in Agaricus, etc. promotes wound healing and, as stated in this report, we believe β-glucan also contributes to wound healing.

Conclusion

It was suggested that consumption of KAOD could be beneficial toward repairing surgical wounds or external wounds, therefore we can anticipate its contribution in veterinary field moving forward.